QUOTE (bjanes @ Aug 1 2008, 06:53 AM)
When you take closeups, you have to extend the lens to achieve focus, and the effective aperture decreases (f/number gets larger). Ne = N*(1+M), where Ne is the effective aperture, M is the magnification, and N is the marked f/number. If you are at a magnification of 1, you lose 2 f/stops.
See the
Lens Faq. Many newer lenses are equipped with microchip so that the actual f/stop is indicated on the camera. TTL metering takes the needed increase in exposure into account.
Bill
Won't that simply make the diffraction worse? You're saying that the effective f-number is larger than 32.
%%%%%%%%%%%%%%%%%%%%%%%%%%%%
It looks as though at f32 you are close to losing resolution of the white dots in the big "C" at upper left; how big across are those in pixels?
The photozone tests of the Canon 100mm/2.8 macro give about 1000 lines/picture height on APS-C at f32. That works out to about 35 line pairs/mm resolved, or a resolved line spacing of about 30µ. That's to be compared with the "size" of the diffraction spot which is ~40µ. So, not far off.
A further issue to consider is whether the size of the Airy disk is the limit of resolution. The disk radius is the location of the first zero of the diffraction intensity pattern. I plotted the intensity pattern of two diffraction spots of increasing separation -- 4, 5, 6, and 8 reading from left to right and top to bottom:

In these somewhat arbitrary units, the first minimum of the diffraction pattern is at a radius of about 3.8. The black circles are at the radius of the Airy disk, centered on each spot.
So clearly at a separation of the imaged spots equal to the diameter of the Airy disk (about 7.8 in the above plots, essentially the lower right figure) the objects are completely resolved. But I also imagine I'd be able to make them out at a separation of 6, the lower left figure; 5 (upper right) is starting to be merged, and 4 (upper left) shows basically one spot.
This is consistent with the Photozone tests, which show a resolution down to about 3/4 the Airy disk diameter.